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Iptg And Repressor
pipetted & spread 100 uL of 0.1 mM IPTG onto AMP plates, let dry. Picked a J04450 colony and streaked it on two plates 30 min later. Looking for induction of the RFP in J04450 via IPTG repression of the LacI repressor.
iptg and repressor
We found that phenylalanine (similarly to tryptophan) affords a functional repressor that is still responsive to IPTG. Characterisation of the W220F mutant, LacIWF, by measuring the time dependence of GFP production at& ...
Lac repressor ceased to be a hypothetical molecule in 1966 when Gilbert and Muller-Hill announced its isolation (5). They used equilibrium dialysis with 14C-labeled inducer IPTG as a test. They had assumed that ten monomeric repressors& ...
At this point, I sequences under the control of the startup sequence in the PI expressed Lac repressor protein sequences combined with O, and P hinder RNA polymerase binding sequence, inhibition of transcription start.
pipetted & spread 100 uL of 0.1 mM IPTG onto AMP plates, let dry. Picked a J04450 colony and streaked it on two plates 30 min later. Looking for induction of the RFP in J04450 via IPTG repression of the LacI repressor.
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